3,3',5,5'-Tetramethylbenzidine
- CasNo：54827-17-7
- Molecular Formula：C16H20N2
- Purity：
- Molecular Weight：
Product Details
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54827-17-7 Name |
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Name |
3,3',5,5'-Tetramethylbenzidine |
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Synonym |
Tetramethylbenzidine;1’-biphenyl)-4,4’-diamine,3,3’,5,5’-tetramethyl-(;1’-biphenyl)-4,4-diamine,3,3’,5,5’-tetramethyl-(;1’-biphenyl]-4,4’-diamine,3,3’,5,5’-tetramethyl-[;TMB-E;TMB EASY;TMB/H;TMB-B;TMB BLUE ONE-STEP SUBSTRATE SYSTEM |
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54827-17-7 Biological Activity |
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Description |
3,3’,5,5’-Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). |
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Related Catalog |
Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others |
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54827-17-7 Chemical & Physical Properties |
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Melting point |
168-171 °C(lit.) |
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Boiling point |
368.6±37.0 °C at 760 mmHg |
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Density |
1.1±0.1 g/cm3 |
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Molecular Formula |
C16H20N2 |
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Molecular Weight |
240.343 |
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Flash Point |
210.8±26.0 °C |
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PSA |
52.04000 |
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LogP |
3.40 |
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Exact Mass |
240.162643 |
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Vapour Pressure |
0.0±0.8 mmHg at 25°C |
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Index of Refraction |
1.618 |
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Storage condition |
2-8°C |
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Stability |
Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents. |
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Water Solubility |
Slightly soluble. |
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54827-17-7 Description |
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Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. Tetramethylbenzidine is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures. |
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54827-17-7 Uses |
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3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content. |
